US 2012/0073018 A1Published application

Jatropha curcas curcin genes, tissue-specific promoters and generation of curcin-deficient transgenic jatropha plants

AssigneeTemasek Life Sciences Laboratory Limited

InventorsZhong Chao Yin, Li Fang Wu, Hui Zhu Mao, Cheng Xiang Qiu

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Abstract

The present invention relates to the isolation of Jatropha curcas curcin genes and tissue-specific promoters and to the production of curcin-deficient Jatropha plants. More specifically, the present invention relates to the isolation of Jatropha curcas Curcin 1, Curcin 2 and Curcin 2 A. The present invention further relates to of the Curcin 1, Curcin 2 A and Curcin 2 genes and more particularly to tissue specific promoters of the Curcin 1 and Curcin 2A genes. The present invention further relates to production of curcin-deficient transgenic jatropha plants by using RNAi technology to suppress curcin gene expression.

Claims28

Dependent claims

An isolated nucleic acid having promoter activity in a plant selected from the group consisting of:

(a) a nucleic acid comprising nucleotides 1 to 3181 of SEQ ID NO:
7;
(b) a nucleic acid comprising nucleotides 1 to 2888 of SEQ ID NO:
7;
(c) a nucleic acid comprising nucleotides 1142 to 3181 of SEQ ID NO:
7;
(d) a nucleic acid comprising nucleotides 1 to 3181 of SEQ ID NO:
7 with nucleotides 2944 to 3170 deleted;
(e) a nucleic acid comprising nucleotides 1142 to 3181 of SEQ ID NO:
7 with nucleotides 2944 to 3170 deleted;
(f) a nucleic acid comprising nucleotides 2688 to 3181 of SEQ ID NO:
7 with nucleotides 2944 to 3170 deleted;
(g) a nucleic acid comprising nucleotides 1 to 2087 of SEQ ID NO:
8;
(h) a nucleic acid comprising nucleotides 912 to 2087 of SEQ ID NO:
8;
(i) a nucleic acid comprising nucleotides 1 to 2087 of SEQ ID NO:
8 with nucleotides 1853 to 2076 deleted;
(j) a nucleic acid comprising nucleotides 912 to 2087 of SEQ ID NO:
8 with nucleotides 1853 to 2076 deleted;
(k) a nucleic acid comprising nucleotides 1751 to 2087 of SEQ ID NO:
8 with nucleotides 1853 to 2076 deleted;
and
(l) a nucleic acid comprising nucleotides 1 to 3465 of SEQ ID NO:
9.

22 more elements

Dependencies are inferred from the claim text.

Facts

Application: 13/375,594Filed 2 Jun 2010
Priority: 5 Jun 2009
Published: 22 Mar 2012
Term ends: Not estimatedThe source records don’t support an estimate.

Classification11 codes

C07K 14/415
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from plants
C12N 15/8218
Methods for controlling, regulating or enhancing expression of transgenes in plant cells: antisense, co-suppression, viral induced gene silencing…
C12N 15/8225
Vegetative tissue-specific promoters: leaf-specific, e.g. including petioles, stomata
C12N 15/8234
Reproductive tissue-specific promoters: seed-specific, e.g. embryo, endosperm