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US 2012/0073015 A1Published application
Methods for plant transformation using spectinomycin selection
AssigneeMonsanto Technology LLC
InventorsBrian Martinell, Michael Petersen, David Somers, Yuechun Wan, Edward Williams, Xudong Ye
Life of this patent
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Abstract
The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
Claims56
-5. (canceled)
A method of producing a transgenic plant comprising:
- (a) transforming at least a first seed explant with a heterologous nucleic acid sequence comprising a selectable marker conferring tolerance to spectinomycin;
- and
- (b) regenerating a transgenic plant from the transformed cells, wherein the explant is contacted, prior to, concurrently with, and/or following step (a) or step (b), with at least a first media comprising spectinomycin to select transformed cells comprising said selectable marker.
-9. (canceled)
(canceled)
-64. (canceled)
Dependencies are inferred from the claim text.