Loading patent…
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsLynn MacDonald, Sean Stevens, Cagan Gurer, Andrew J. Murphy, Karolina A. Hosiawa
Estimating the term…
Genetically modified mice are provided that express human λ variable (hVλ) sequences, including mice that express hVλ sequences from an endogenous mouse λ light chain locus, mice that express hVλ sequences from an endogenous mouse κ light chain locus, and mice that express hVλ sequences from a transgene or an episome wherein the hVλ sequence is linked to a mouse constant sequence. Mice are provided that are a source of somatically mutated human λ variable sequences useful for making antigen-binding proteins. Compositions and methods for making antigen-binding proteins that comprise human λ variable sequences, including human antibodies, are provided.
A mouse, comprising:
A mouse comprising:
A mouse that expresses an antibody comprising a light chain that comprises a λ variable sequence and a κ constant sequence.
An immunoglobulin light chain, comprising a human λ variable region and a mouse κ constant region.
A cell that expresses an immunoglobulin light chain that comprises a human λ variable region fused with a mouse κ constant region.
A method for making a human λ variable region nucleic acid sequence, comprising a step of cloning from a cell a human λ variable region nucleic acid sequence, wherein the cell is derived from a mouse that comprises in its germline a hVλ gene segment and a hJλ gene segment operably linked to a mouse κ constant region nucleic acid sequence at an endogenous mouse κ constant light chain locus.
Dependencies are inferred from the claim text.