Methods of producing laser speckle contrast images

A method comprising:

• obtaining a raw speckle image of a sample;
• converting the raw speckle image to a laser speckle contrast image using a roll algorithm that uses the following formula:
• k
• =
• s
• I
• 〈
• I
• 〉
• =
• N
• 
• ∑
• i
• =
• 1
• N
• 
• I
• i
• 2
• -
• (
• ∑
• i
• =
• 1
• N
• 
• I
• i
• )
• 2
• N
• 
• (
• N
• -
• 1
• )
• ∑
• i
• =
• 1
• N
• 
• I
• i
• N
• where speckle contrast k is seen to be equal to the standard deviation of time-integrated intensity sI divided by the mean time-integrated intensity I and where N is a positive integer;
• and
• converting the laser speckle contrast image to a relative correlation time image using a relative correlation time algorithm.

A method comprising:

• obtaining a raw speckle image of a sample;
• converting the raw speckle image to a laser speckle contrast image using a laser speckle contrast algorithm;
• and
• converting the laser speckle contrast image to a relative correlation time image using a relative correlation time algorithm selected from the group consisting of a Newton method algorithm, a table method algorithm, a hybrid method algorithm, or an asymptote method algorithm.

A laser speckle contrast imaging system comprising:

• a laser light source;
• a detector for the measurement of reflected light comprising at least one camera and at least one magnification objective;
• a microprocessor or data acquisition unit;
• and
• a memory, the memory including executable instructions that, when executed, cause the microprocessor or data acquisition unit to convert a raw speckle image to a laser speckle contrast image using a laser speckle contrast algorithm.