System and methods for purifying biological materials

A fluid sample purification system for isolating a molecule of interest in a fluid sample comprising:

• a housing having a distal end with a distal opening adapted for the passage of a fluid and a proximal end with a proximal opening adapted for passage of a fluid;
• a distal retainer inside the housing and above the distal opening;
• a proximal retainer, wherein the proximal retainer is located:
• inside the housing and between the distal retainer and the proximal opening, or adjacent to, in contact with, covering or sealing the proximal opening;
• and
• adsorption material comprising a plurality of functionalized particles, wherein:
• the plurality of functionalized particles is inside the housing and confined between the distal retainer and the proximal retainer, and
• the functionalized particles of the plurality adsorb undesirable material.

A method for isolating a molecule of interest in a fluid sample comprising the steps of:

• a. providing a fluid sample purification system, wherein the system comprises:
• a housing having a distal end with a distal opening adapted for the passage of a fluid and a proximal end with a proximal opening adapted for passage of a fluid;
• adsorption material comprising a plurality of functionalized particles;
• and
• a distal retainer and proximal retainer for retaining the adsorption material;
• b. drawing a fluid sample into the fluid sample purification system;
• c. contacting the plurality of functionalized particles with the fluid sample;
• and
• d. aspirating and dispensing the fluid sample using an aspirating/dispensing cycle and fluid flow rate that maintains a consistent and consistently high rate of fluid flow, thereby expelling the fluid sample from the fluid sample purification system.