Sulfurizing reagents and their use for oligonucleotides synthesis

Abstract

An oligonucleotide which comprises at least one internucleotide linkage comprising a P—S—R bond and at least two nucleosides, wherein R corresponds to the formula (I) wherein A is a geminally substituted alkylene group, preferably CH2, X and Y are independently selected from S and O, and R0 is selected from the group consisting of optionally substituted carbon bonded organic residue, such as in particular optionally substituted alkyl or aryl, SRx, ORx and NRxRy wherein Rx and/or Ry are selected from H and organic residues and at least Rx is a substituent other than H. Another object of the invention is a sulfurizing agent useful for oligonucleotide manufacture and the manufacture thereof. Other nucleotides described comprise at least one internucleotide linkage comprising a P—S—R bond, at least one internucleotide linkage comprising a P—S—R′ bond and at least three nucleosides wherein R′ is an organic residue other than the group R, preferably selected from a group consisting of an aryl group and a heteroaryl group which is bonded to the S-atom through an annular carbon atom.

Dependencies are inferred from the claim text.