Selective enrichment of antibodies
AssigneeBoehringer Ingelheim International GmbH
InventorsDorothee AMBROSIUS, Michael DIETERLE, Philine DOBBERTHIEN, Maria-Katharina HOYER
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Abstract
The invention relates to a process for the selective concentration of immunoglobulins or other proteins that contain an Fc domain (target protein), comprising the following steps: a. preparing a solution that contains the target protein; b. incorporating an Fc-binding protein with precisely two binding sites under conditions that allow binding to occur; c. separating the precipitate from the liquid phase; d. undoing the binding of the target protein from the Fc-binding protein.
Claims11
Process for the selective concentration of immunoglobulins or other proteins that contain an Fc domain (target protein), comprising the following steps:
- a. preparing a solution that contains the target protein;
- b. incorporating an Fc-binding protein with precisely two binding sites under conditions that allow binding to occur;
- c. separating the precipitate from the liquid phase;
- d. undoing the binding of the target protein from the Fc-binding protein.
Fc-binding protein that consists of 2 identical sub-units that have the sequence SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, or a sequence that differs from SEQ ID NO. 1 in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 amino acids, wherein the two sub-units are linked together via a covalent bond.
Dependencies are inferred from the claim text.