Method for purification of target polypeptides
AssigneeNovozymes A/S
InventorsJan Khyse-Andersen, Lars Winther
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Abstract
The present invention relates to a process for purification of a target molecule, comprising the steps: (a) contacting a target molecule, and a population of target binding polypeptides (TBP), in solution for a sufficient time to allow complex formation; and (b) isolating the target from the complex from (a) by subsequent purification steps, wherein (i) the target binding polypeptides have at least two binding functionalities; a first binding functionality towards the target and a second binding functionality towards a catching ligand comprised in a solid support; and (ii) the first binding functionality comprises at least two binding sites for the target, and the target comprises at least two binding sites for the TBP.
Claims23
A process for purification of a target molecule, comprising the steps:
- (a) contacting a target molecule, and a population of target binding polypeptides (TBP), in solution for a sufficient time to allow complex formation;
- and (b) isolating the target from the complex from (a) by subsequent purification steps, wherein (i) the target binding polypeptides have at least two binding functionalities;
- a first binding functionality towards the target and a second binding functionality towards a catching ligand comprised in a support;
- and (ii) the first binding functionality comprises at least two binding sites for the target, and the target comprises at least two binding sites for the TBP.
A TBP for purification of a target molecule, wherein (i) the target binding polypeptide have at least two binding functionalities;
- a first binding functionality towards the target and a second binding functionality towards a catching ligand comprised in a support;
- and (ii) the first binding functionality comprises at least two binding sites for the target.
Dependencies are inferred from the claim text.