Appoptosin and uses thereof for treating neurodegenerative disease and cancer
AssigneesTsinghua University, Sanford-Burnham Medical Research Institute
InventorsHuaxi Xu, Francesca-Fang Liao, Han Zhang, Yunwu Zhang, Fangfang Zhou, Yeguang Chen
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Abstract
Disclosed herein are compositions and methods relating to the modulation of Appoptosin levels or activity in the treatment of Neurodegenerative disorders or cancer.
Claims18
1-30. (canceled)
An isolated nucleic acid comprising
- (a) a nucleic acid sequence encoding the amino acid sequence set forth in SEQ ID NO:2;
- (b) a nucleic acid sequence encoding a fragment of the amino acid sequence set forth in SEQ ID NO:2, wherein the fragment binds to the amyloid precursor protein (APP) intracellular domain (AICD);
- (c) a nucleic acid sequence encoding an amino acid sequence having at least about 70, 75, 80, 85, 90, 95%, or 100% sequence identity to the amino acid sequence set forth in SEQ ID NO:2, wherein the polypeptide binds to the APP AICD;
- or
- (d) a nucleic acid sequence encoding an amino acid sequence having at least about 70, 75, 80, 85, 90, 95%, or 100% sequence identity to a fragment of the amino acid sequence set forth in SEQ ID NO:2, wherein the polypeptide binds to the APP AICD.
The nucleic acid of claim 1, wherein the fragment of the amino acid sequence set forth in SEQ ID NO:2 comprises the amino acid sequence set forth in amino acids 118 to 264 of SEQ ID NO:2.
The nucleic acid of claim 1, wherein the fragment of the amino acid sequence set forth in SEQ ID NO:2 comprises the amino acid sequence set forth in amino acids 264 to 304 of SEQ ID NO:2.
The nucleic acid of claim 1, wherein the amino acid sequence has at least about 70, 75, 80, 85, 90, 95%, or 100% sequence identity to the amino acid sequence set forth in amino acids 118 to 264 of SEQ ID NO:2.
The nucleic acid of claim 1, wherein the amino acid sequence has at least about 70, 75, 80, 85, 90, 95%, or 100% sequence identity to the amino acid sequence set forth in amino acids 264 to 304 of SEQ ID NO:2.
The nucleic acid of claim 1, wherein the fragment consists of a conservative variant of the amino acid sequence set forth in amino acids 264 to 304 of SEQ ID NO:2.
The nucleic acid sequence of claim 1, wherein the fragment consists of the amino acid sequence set forth in amino acids 118 to 264 of SEQ ID NO:2.
The nucleic acid sequence of claim 1, wherein the fragment consists of the amino acid sequence set forth in amino acids 264 to 304 of SEQ ID NO:2.
The nucleic acid of claim 1, wherein the nucleic acid comprises the nucleic acid sequence set forth in SEQ ID NO:1.
An isolated polypeptide comprising
- (a) the amino acid sequence set forth in SEQ ID NO:2;
- (b) a fragment of the amino acid sequence set forth in SEQ ID NO:2, wherein the fragment binds to the APP AICD;
- (c) an amino acid sequence having at least about 70, 75, 80, 85, 90, 95%, or 100% sequence identity to the amino acid sequence set forth in SEQ ID NO:2, wherein the polypeptide binds to the APP AICD;
- or
- (d) an amino acid sequence having at least about 70, 75, 80, 85, 90, 95%, or 100% sequence identity to a fragment of the amino acid sequence set forth in SEQ ID NO:2, wherein the polypeptide binds to the APP AICD.
The polypeptide of claim 10, wherein the fragment of the amino acid sequence set forth in SEQ ID NO:2 comprises the amino acid sequence set forth in amino acids 118 to 264 of SEQ ID NO:2.
The polypeptide of claim 10, wherein the fragment of the amino acid sequence set forth in SEQ ID NO:2 comprises the amino acid sequence set forth in amino acids 264 to 304 of SEQ ID NO:2.
The polypeptide of claim 10, wherein the amino acid sequence has at least about 70, 75, 80, 85, 90, 95%, or 100% sequence identity to the amino acid sequence set forth in amino acids 118 to 264 of SEQ ID NO:2.
The polypeptide of claim 10, wherein the amino acid sequence has at least about 70, 75, 80, 85, 90, 95%, or 100% sequence identity to the amino acid sequence set forth in amino acids 264 to 304 of SEQ ID NO:2.
The polypeptide of claim 10, wherein the fragment consists of a conservative variant of the amino acid sequence set forth in amino acids 264 to 304 of SEQ ID NO:2.
The polypeptide of claim 10, wherein the fragment consists of the amino acid sequence set forth in amino acids 118 to 264 of SEQ ID NO:2.
The polypeptide of claim 10, wherein the fragment consists of the amino acid sequence set forth in amino acids 264 to 304 of SEQ ID NO:2.
Dependencies are inferred from the claim text.