METHODS OF QUANTIFYING N2-(1-CARBOXYETHYL)-2'-DEOXY-GUANOSINE (CEdG) AND SYNTHESIS OF OLIGONUCLEOTIDES CONTAINING CEdG
OwnerSamuel Rahbar, Timothy W. Synold +1inventors
InventorsSamuel Rahbar, Timothy W. Synold, John Termini
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Abstract
Methods of quantifying a N2-carboxyethyl-2′-deoxyguanosine (CEdG) levels in biological samples and comparing those levels to known normal levels can diagnose a number of disorders, including diabetes and cancer. Methods can also determine whether therapies for disorders are effective by measuring CEdG levels before and after treatment. Measurement of CEdG levels occurs using liquid chromatography electrospray ionization tandem mass spectrometry.
Claims16
A method of quantifying N2-carboxyethyl-2′-deoxyguanosine (CEdG) in a tumor tissue sample, comprising:
- performing a liquid chromatography electrospray ionizing tandem mass spectrometry assay on a tumor tissue sample from a subject using a stable isotope dilution and an internal 15N5-CEdG standard comprising oligonucleotides containing stereochemically pure (R) or (S) 15N5-CEdG;
- wherein the assay determines the quantity of CEdG in the tumor tissue sample.
A method of detecting cancer in a subject comprising:
- quantifying a CEdG level in a biological sample from a subject using liquid chromatography electrospray ionization tandem mass spectrometry and an internal CEdG standard comprising oligonucleotides containing stereochemically pure (R) or (S) 15N5-CEdG;
- wherein a lower level of CEdG as compared to a normal physiological CEdG level indicates that the subject has a glycolytic cancer.
Dependencies are inferred from the claim text.