Methods of inhibiting cancer cell growth with hdac inhibitors and methods of screening for hdac10 inhibitors
AssigneeDuke University
InventorsTso-pang Yao, Hitoshi Sasajima, Yoshiharu Kawaguchi, Kai Cui, Chun-Hsiang Lai
Life of this patent
Estimating the term…
Abstract
Methods of inhibiting cancer cell growth using HDAC10 inhibitors are provided. Methods of treating cancer in a subject using HDAC10 inhibitors are also provided. In certain embodiments, at least one second inhibitor selected from an autophagy inhibitor, an AMPK inhibitor, and methyl pyruvate is also used in the methods. Dose packs comprising HDAC10 inhibitors and at least one second inhibitor are provided. Methods of identifying HDAC10 inhibitors are also provided.
Claims26
A method of inhibiting cancer cell growth comprising contacting the cancer cell with at least one HDAC10 inhibitor and at least one second inhibitor selected from an autophagy inhibitor, an AMPK inhibitor, and methyl pyruvate.
(canceled)
-17. (canceled)
A method of identifying a HDAC10 inhibitor comprising:
- (a) contacting cells in vitro with a test agent, and
- (b) comparing the acetylation level of a VDAC in the contacted cells of step (a) to the acetylation level of a VDAC in control cells,
- wherein an increase in the acetylation level of a VDAC in the contacted cells of step (a) relative to the acetylation level of a VDAC in the control cells indicates that the test agent is an HDAC10 inhibitor.
(canceled)
(canceled)
A method of treating cancer comprising administering to a subject at least one HDAC10 inhibitor and at least one second inhibitor selected from an autophagy inhibitor, an AMPK inhibitor, and methyl pyruvate.
(canceled)
-39. (canceled)
Dependencies are inferred from the claim text.