Back to results
US 2012/0071361 A1Published application
Substrate Preparation Process
AssigneeAffymetrix, Inc.
InventorsMartin Goldberg, Martin Diggelman, Earl Hubbell, Glenn McGall, Nam Quoc Ngo, MacDonald Morris, Mel Yamamoto, Jennifer Tan, Richard P. Rava
Life of this patent
Estimating the term…
Abstract
The present invention provides novel processes for the large scale preparation of arrays of polymer sequences wherein each array includes a plurality of different, positionally distinct polymer sequences having known monomer sequences. The methods of the invention combine high throughput process steps with high resolution photolithographic techniques in the manufacture of polymer arrays.
Claims27
-7. (canceled)
A system for synthesizing an array of polymers, the system comprising:
- a flow cell, wherein the flow cell comprises a flow cell body and a substrate, wherein the flow cell body and the substrate are configured to form a flow cell cavity, wherein the substrate includes an array synthesis surface within the flow cell cavity, and wherein the flow cell cavity includes an anti-reflective coating;
- one or more fluidic ports configured to flow one or more fluids through the flow cell cavity;
- and
- a light source configured to illuminate the array synthesis surface.
1 more element
A system for synthesizing an array of polymers, the system comprising:
- a flow cell, wherein the flow cell comprises a flow cell body and a substrate, wherein the flow cell body and the substrate are configured to form a flow cell cavity, wherein the substrate includes an array synthesis surface within the flow cell cavity, wherein the flow cell cavity includes a back surface, wherein the back surface is located opposite the array synthesis surface, and wherein the back surface possesses an anti-reflective coating;
- one or more fluidic ports configured to flow one or more fluids through the flow cell cavity;
- a fluid delivery system, wherein the fluid delivery system is configured to be in fluid communication with the flow cell cavity through the one or more fluidic ports, and wherein the fluid delivery system comprises a plurality of reagent reservoirs;
- and
- a light source configured to illuminate the array synthesis surface from a side of the substrate which is opposite the array synthesis surface such that illumination passes through the substrate before reaching the array synthesis surface, wherein the anti-reflective coating reduces reflection of one or more wavelengths off of the back surface, and wherein the one or more wavelengths include a wavelength above 340 nm.
2 more elements
A method of synthesizing an array of polymers, the method comprising:
- (a) providing a substrate, wherein the substrate includes an array synthesis surface, and wherein the array synthesis surface comprises functional groups protected by photolabile protecting groups;
- (b) deprotecting the functional groups within selected regions of the array synthesis surface with illumination configured to remove the photolabile protecting groups and produce exposed functional groups, wherein the illumination occurs within a synthesis system, wherein the synthesis system comprises a light source and an anti-reflective coating, and wherein the anti-reflective coating reduces reflection of one or more wavelengths of the illumination within the synthesis system;
- (c) coupling monomers to the exposed functional groups within the selected regions, wherein the monomers are protected by photolabile protecting groups;
- and
- (d) repeating steps (b)-(c) for one or more repetitions with different sets of selected regions to synthesize the array of polymers.
2 more elements
A method of synthesizing an array of polymers, the method comprising:
- (a) providing a synthesis system, wherein the synthesis system comprises a light source and a flow cell, wherein the flow cell comprises a flow cell body and a substrate, wherein the flow cell body and the substrate form a flow cell cavity, wherein the substrate includes an array synthesis surface within the flow cell cavity, wherein the flow cell cavity includes a back surface, wherein the back surface is located opposite the array synthesis surface within the flow cell cavity, wherein the back surface possesses an anti-reflective coating, and wherein the array synthesis surface comprises functional groups protected by photolabile protecting groups;
- (b) deprotecting the functional groups within selected regions of the array synthesis surface with illumination configured to remove the photolabile protecting groups and produce exposed functional groups, wherein the light source illuminates the selected regions from a side of the substrate which is opposite the array synthesis surface such that the illumination passes through the substrate before reaching the array synthesis surface, and wherein the anti-reflective coating reduces reflection of one or more wavelengths off of the back surface;
- (c) coupling monomers to the exposed functional groups within the selected regions, wherein the monomers are protected by photolabile protecting groups;
- and
- (d) repeating steps (b)-(c) for one or more repetitions with different sets of selected regions to synthesize the array of polymers.
2 more elements
Dependencies are inferred from the claim text.