Fluidic devices and methods for multiplex chemical and biochemical reactions

A method for amplifying one or more target nucleic acids:

• (a) attaching an oligonucleotide to a solid support within a chamber, the oligonucleotide comprising a first primer, a second primer and a binding probe sequence wherein the first primer, second primer and binding probe sequence are separated from one another and the solid support by a cleavable linker;
• (b) incubating the target nucleic acid(s) comprising with the oligonucleotide under conditions in which complementary target nucleic acid sequences and binding probe sequences hybridize to one another;
• (c) washing the chamber;
• (d) adding a solution comprising a cleavage substance, polymerase, dNTPs, and divalent cation to the chamber such that the first primer, second primer, and binding probe sequence are released from one another and from the solid support so that the first primer, second primer, binding probe sequence, target nucleic acid, polymerase, dNTPs and divalent cation produce a reaction mixture within the;
• (e) subjecting the reaction mixture to two or more cycles of heating and cooling such that the target nucleic acids are amplified.

A method for amplifying a plurality of target nucleic acids on a microarray wherein the microarray is comprised of a plurality of separate chambers comprising:

• (a) attaching an first oligonucleotide to a solid support within a first chamber, the oligonucleotide comprising a first primer, a second primer and a first binding probe sequence wherein the first primer, second primer and binding probe sequence are separated from one another and the solid support by a cleavable linker;
• (b) attaching a second oligonucleotide to a solid support within a second chamber, the second oligonucleotide comprising a third primer, a fourth primer and a second binding probe sequence wherein the third primer, fourth primer and second binding probe sequence are separated from one another and the solid support by a cleavable linker;
• (c) incubating a target nucleic acid comprising two or more nucleic acid sequences with the first and second oligonucleotide under conditions in which complementary target nucleic acid sequences and binding probe sequences hybridize to one another;
• (d) washing the first and second chambers;
• (e) adding a solution comprising a cleavage substance, polymerase, dNTPs, and divalent cation to the first and second chamber such that the first primer, second primer, third primer, fourth primer, first binding probe sequence and second binding probe sequence are released from one another and from the solid support so that the first primer, second primer, first binding probe sequence, target nucleic acid, polymerase, dNTPs and divalent cation produce a first reaction mixture within the first chamber and the third primer, fourth primer, second binding probe sequence, target nucleic acid, polymerase, dNTPs and divalent cation produce a second reaction mixture within the second chamber;
• (f) subjecting the first and second reaction mixture to two or more cycles of heating and cooling such that a plurality of target nucleic acids are amplified.