Methods and systems for uniform enrichment of genomic regions
OwnerJacob Kitzman, Todd Richmond +4inventors
InventorsJacob Kitzman, Todd Richmond, Mark D'Ascanzo, Thomas Albert, Jeffrey Jeddeloh, Christina Middle
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Abstract
The present invention provides methods and compositions for the enrichment of target nucleic acids in a microarray system. In particular, the present invention provides methods and compositions for uniform enrichment of target nucleic acid molecules in a microarray format. The present invention also provides for intentionally non-uniform enrichment among target nucleic acid molecules.
Claims2
A composition comprising a solid support and a plurality of nucleic acid probes immobilized on said solid support, wherein each of said plurality of immobilized nucleic acid probes provides for uniform hybridization among a plurality of target nucleic acid sequences, and wherein the frequency of individual immobilized nucleic acid probe sequences corresponds to the frequency of the corresponding plurality of target nucleic acid sequences.
A kit for performing uniform enrichment of target nucleic acid sequences comprising one or more containers, wherein said one or more containers comprises:
- a) a solid support comprising immobilized nucleic acid probes, wherein said probes are selected from a group consisting of a plurality of probes hybridizable to a plurality of target nucleic acid sequences and wherein said probes provide for uniform enrichment of said plurality of target nucleic acid sequences, and
- b) one or more reagents for performing hybridizations, washes, and elution of target nucleic acid sequences.
Dependencies are inferred from the claim text.