Label free biosensors and cells
AssigneeCorning Incorporated
InventorsYe Fang, Ann M. Ferrie, Norman H. Fontaine, Joydeep Lahiri, Po Ki Yuen
Life of this patent
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Abstract
Disclosed are compositions and methods for using label free optical biosensors for performing cell assays. In certain embodiments the assays can be performed in highthough put methods and can be multiplexed.
Claims11
-270. (canceled)
A method to test the effect of a stimulatory event on a cell comprising:
- providing a RWG (Resonant Wave Guide Grating) label-free biosensor;
- incubating a cell on the biosensor;
- providing a stimulatory event to the incubated cell;
- collecting from within the cell, at the cell surface, or a combination thereof, the DMR (Directional Mass Redistribution) biosensor output from the biosensor;
- analyzing the DMR to determine the effect of the stimulatory event on the cell;
- and detecting a label with a device capable of detecting the label.
-277. (canceled)
A method of identifying a state of a cell, the method comprising:
- culturing the cell on a surface of a label free-biosensor to form a cell-biosensor composition;
- and
- assaying the cell-biosensor composition, where the assaying comprises identifying an inhomogeneity at the surface where the cell is cultured.
A method of determining a state of a living cell comprising observing the dynamic mass redistribution of the cellular contents with a resonant waveguide grating biosensor.
A method for testing a compound's effect on a cell comprising:
- culturing cells on a biosensor;
- washing the cells;
- starving the cells;
- incubating the compound with the cell;
- and
- recording a signal with a biosensor.
Dependencies are inferred from the claim text.