Novel anti-aging agents and methods to identify them
OwnerHaiyan Qiinventor
Life of this patent
Estimating the term…
Abstract
The present invention discloses novel mechanisms in the aging process and describes novel methods for high-throughput screening to identify, detect, and purify agents to be used for improving mitochondrial function, maintaining the cell cycle-arrested state in senescent and post mitotic cells, and thus preventing or treating age-related diseases or disorders associated with accelerated mitochondrial function loss, telomere dysfunction, and/or deterioration of the growth-arrested state. The present invention also discloses a number of compounds or compositions identified from this method. The present invention further provides the use of low doses of rapamycin or its analogs as a mimic of caloric restriction in preventing age-related diseases or disorders.
Claims24
A method of identifying or detecting an agent for preventing or treating an age-related disease or disorder, the method comprising screening one or more compounds or compositions against a senescence model system and monitoring their anti-aging activity.
A method for detecting an anti-aging agent or determining biological concentration of an anti-aging agent in a biological sample, the method comprising the steps of:
- (i) optionally diluting a subject sample with a solvent;
- (ii) incubating the sample with mutant yeast cells under conditions where the cell cycle is arrested by telomere dysfunction or DNA damage;
- (iii) removing the conditions under which the cell cycle is arrested and measuring the number of survived yeast cells;
- and
- (iv) comparing the number of survived cells obtained in step (iii) to the number of survived cells in a control experiment under the same conditions as those of the incubating step (ii) except for the absence of the subject sample,
- wherein the increased number of survived cells obtained in step (iii) compared to the number of survived cells in the control experiment indicates that the biological sample comprises an anti-aging agent.
Dependencies are inferred from the claim text.