Methods for large scale functional evaluation of nucleotide sequences in plants

A method of analyzing plants having a phenotype of interest comprising:

• obtaining a population of plants grown under greenhouse conditions,
• obtaining phenotypic characteristics of the population of plants,
• comparing phenotypic characteristics of plants in the population to one another to
• identify phenotypic differences among the plants,
• identifying plants having a phenotype of interest, and
• selecting plants with said phenotype of interest.

A high throughput method of assessing a plant trait of interest comprising:

• providing a population of plants with different genotypes,
• growing the plant population under greenhouse conditions,
• analyzing individual plants having the trait,
• comparing plants having the trait to other plants in the provided population, to identify the genetic basis of the trait.

A method of selecting plants having a phenotype of interest comprising:

• obtaining a population of plants grown under greenhouse conditions,
• visually screening individual plants in the plant population to identify phenotypic characteristics,
• comparing plants in the screened population to one another to identify phenotypic differences among the plants,
• identifying plants having a phenotype of interest, and
• selecting plants with said phenotype of interest.

A high throughput method of assessing a plant trait of interest comprising:

• obtaining a population of plants with different genetic constitutions,
• growing the plant population under greenhouse conditions,
• visually screening individual plants having the trait,
• comparing plants having the trait to other plants in the obtained population, to identify the genetic basis of the trait.

A method of analyzing plants expressing a target gene the method comprising:

• obtaining a population of plants grown under greenhouse conditions,
• obtaining phenotypic characteristics of the population of plants,
• comparing phenotypic characteristics of the plants,
• analyzing phenotypic differences among the plants;
• and
• identifying plants that are relevant to the trait of interest.

A method of analyzing transgenic plants expressing a trait of interest:

• obtaining plant cells for transformation,
• obtaining a plurality of nucleotide vectors,
• introducing the plurality of vectors to form a transgenic event population comprising transgenic plants,
• growing the transgenic plants under greenhouse conditions,
• visually screening individual plants having the trait;
• and
• identifying plants that are relevant to the trait of interest.