Indexing of nucleic acid populations
AssigneeFebit Holding GmbH
InventorsPeer F. Staehler, Cord F. Staehler, Markus Beier, Mark S. Chee, Nadine Schracke
Life of this patent
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Abstract
The invention relates to a method for acquisition of genetic information, in particular for personalized medicine.
Claims16
A method for isolation of target nucleic acid molecules, comprising the steps:
- (a) providing one or more nucleic acid molecule populations to be analyzed,
- (b) introducing markings into the nucleic acid populations to be analyzed,
- (c) bringing the one or more populations of nucleic acid molecules into contact with capture molecules under conditions under which target nucleic acid molecules from the population or populations to be analyzed can bind specifically to the capture molecules,
- (d) separating off material not bound to capture molecules and
- (e) isolating and optionally characterizing the target nucleic acid molecules, comprising determination of the markings.
An apparatus for acquisition of information in the DNA or RNA of an individual by sequence-specific enrichment of target regions of the DNA or RNA in/on a capture probe matrix, e.g. a preparative biochip, comprising
- a capture probe matrix,
- a device for loading the capture probe matrix with a DNA or RNA sample,
- a device for feeding reagents for washing the capture probe matrix,
- a device for elution of an enriched DNA or RNA sample from the capture probe matrix,
- one or more sequencing reaction chambers,
- a device for loading the one or more sequencing reaction chambers
- a device for carrying out a parallel sequencing reaction in the sequencing reaction chambers, e.g. by means of sequencing-by-synthesis or by means of sequencing-by-ligation,
- a memory-programmable device for carrying out the parallel sequencing reaction,
- a memory-programmable device and a storage medium for storage of the sequencing results.
An apparatus for acquisition of information in the DNA or RNA of an individual by sequence-specific enrichment of target regions of the DNA or RNA in/on a preparative biochip, comprising
- a capture probe matrix,
- a device for loading the capture probe matrix with a DNA or RNA sample,
- a device for feeding reagents for washing the capture probe matrix,
- a device for elution of the enriched DNA or RNA sample from the capture probe matrix,
- one or more sequencing supports,
- a device for loading the one or more sequencing supports in the form of beads, microbeads or microparticles,
- a device for loading a support or a flow cell with the beads, microbeads or microparticles,
- a device for carrying out a parallel sequencing reaction, e.g. by means of sequencing-by-synthesis or by means of sequencing-by-ligation,
- a memory-programmable device for carrying out the parallel sequencing reaction,
- a memory-programmable device and a storage medium for storage of the sequencing results.
Dependencies are inferred from the claim text.