Label-free method for detecting presence or absence of nucleic acids
OwnerXiaodi Su, Roejarek Kanjanawarutinventors
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Abstract
The present invention is directed to a method of detecting presence or absence of a target nucleic acid using negatively charged metallic nanoparticles dissolved in a solution. A nucleic acid probe with a substantially neutral net charge is used for detecting the target nucleic acid. The present invention is also directed to a kit including at least one nucleic acid probe with a substantially neutral net charge and at least one type of negatively charged metallic nanoparticles for carrying out the method.
Claims26
A method of detecting presence or absence of a target nucleic acid, wherein the method comprises:
- providing a nucleic acid probe with a substantially neutral net charge and contacting the nucleic acid probe with a sample which is suspected to comprise the target nucleic acid to result in a first mixture;
- wherein the nucleic acid probe is substantially complementary to the target nucleic acid;
- contacting the first mixture with negatively charged metallic nanoparticles to result in a second mixture;
- determining whether the target nucleic acid is comprised in the second mixture or not.
A method of detecting presence or absence of a target nucleic acid, wherein the method comprises:
- incubating negatively charged metallic nanoparticles with a sample which is suspected to comprise the target nucleic acid to form a first mixture;
- contacting the first mixture with a nucleic acid probe with a substantially neutral net charge to result in a second mixture;
- wherein the nucleic acid probe is substantially complementary to the target nucleic acid;
- determining whether the target nucleic acid is comprised in the second mixture or not.
A kit for detecting presence or absence of a target nucleic acid in a sample;
- wherein the kit comprises:
- at least one nucleic acid probe with a substantially neutral net charge;
- and
- at least one type of negatively charged metallic nanoparticles.
Use of at least one type of negatively charged metallic particle in a method of detecting presence or absence of a target nucleic acid, wherein the method comprises:
- providing a nucleic acid probe with a substantially neutral net charge and contacting the nucleic acid probe with a sample which is suspected to comprise the target nucleic acid to result in a first mixture;
- wherein the nucleic acid probe is substantially complementary to the target nucleic acid;
- contacting the first mixture with negatively charged metallic nanoparticles to result in a second mixture;
- determining whether the target nucleic acid is comprised in the second mixture or not.
Use of at least one nucleic acid probe with a substantially neutral net charge in a method of detecting presence or absence of a target nucleic acid, wherein the method comprises:
- providing a nucleic acid probe with a substantially neutral net charge and contacting the nucleic acid probe with a sample which is suspected to comprise the target nucleic acid to result in a first mixture;
- wherein the nucleic acid probe is substantially complementary to the target nucleic acid;
- contacting the first mixture with negatively charged metallic nanoparticles to result in a second mixture;
- determining whether the target nucleic acid is comprised in the second mixture or not.
Dependencies are inferred from the claim text.