Immuno conjugate and process for preparation thereof
AssigneeCouncil of Scientific and Industrial Research
InventorsChander Raman SURI, Jas Deep Kaur, Kanwar Vikas Singh, Grish Chandra Varshney, Manoj Raje
Life of this patent
Estimating the term…
Abstract
Present invention deals with a new immuno-reagent comprising hapten (MPAD)-protein-gold for pesticides detection. The conjugate MPAD-protein-gold competes with the analyte of interest for a finite number of binding sites provided by anti-atrazine antibodies coated on nitrocellulose membrane. The newly developed conjugate has a long shelf life with high stability at 4° C. The dynamic concentration range for standard atrazine solutions shows a linear inhibition (decrease in intensity of color) between 10 ppb to 1 ppm atrazine in water samples.
Claims20
-21. (canceled)
A method for detecting a pesticide in a sample comprising:
- providing a kit comprising at least one test line comprising an anti-pesticide antibody and at least one control line comprising an anti-carrier protein antibody, wherein the at least one test line and the at least one control line are immobilized in a detection zone;
- contacting the kit with a sample and a conjugate comprising gold, a carrier protein, and a pesticide derivative;
- and detecting the presence of the pesticide in the sample.
Dependencies are inferred from the claim text.