Method to deplete monoclonal antibodies in a biological sample
OwnerMark Pescovitz, Benita Book +1inventors
InventorsMark Pescovitz, Benita Book, Ora Pescovitz
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Abstract
The present invention provides methods for depleting a biological sample from a patient of a humanized or chimeric monoclonal antibody prior to cross-matching the sample for transplantation. The method comprises treating the sample with a peptide or other moiety that selectively binds to the antibody, forming a peptide-antibody complex and removing the complex from the serum sample. These steps may be repeated as many times as needed to reduce the humanized or chimeric monoclonal antibody to the desired level.
Claims20
A method of treating a biological sample from a patient for transplantation cross-matching, wherein the patient had been administered a monoclonal antibody treatment, the method comprising:
- (a) mixing a peptide with the biological sample wherein the peptide selectively binds to said monoclonal antibody;
- (b) incubating the peptide-sample mixture wherein the peptide selectively binds to said monoclonal antibody, forming a peptide-monoclonal antibody complex;
- and
- (c) removing the peptide-monoclonal antibody complex from the biological sample.
A method of treating a biological sample from a patient for transplantation cross-matching, wherein the patient had been administered a monoclonal antibody treatment comprising an anti-CD20 antibody, an anti-CD3 antibody or an anti-CD52 antibody, the method comprising:
- (a) mixing a peptide with the biological sample wherein the peptide selectively binds to said monoclonal antibody;
- (b) incubating the peptide-sample mixture wherein the peptide selectively binds to said monoclonal antibody, forming a peptide-monoclonal antibody complex;
- and
- (c) removing the peptide-monoclonal antibody complex from the biological sample.
A method of treating a biological sample from a patient for transplantation cross-matching, wherein the patient had been administered a monoclonal antibody treatment comprising an anti-CD20 antibody, the method comprising:
- (a) mixing a peptide with the biological sample wherein the peptide selectively binds to said monoclonal antibody;
- (b) incubating the peptide-sample mixture wherein the peptide selectively binds to said monoclonal antibody, forming a peptide-monoclonal antibody complex;
- (c) removing the peptide-monoclonal antibody complex from the biological sample;
- (d) detecting any monoclonal antibody remaining in the serum sample after step (c);
- and
- (e) repeating (a) to (c) if the monoclonal antibody is detected in step (d).
Dependencies are inferred from the claim text.