Method for analysis of sugar chains by mass spectrometry
AssigneeSumitomo Bakelite Co., Ltd.
InventorsHideyuki SHIMAOKA, Midori ABE, Kota IGARASHI
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Abstract
Provided is a highly accurate means for analyzing a sugar chain contained in a biological sample by MALDI-TOF MS or such a mass spectrometry method. In the present invention, it was found to be possible to improve the ionization efficiency of a sugar chain by methylating the hydroxyl groups of the sample sugar chain before conducting the MALDI-TOF MS analysis, and by so doing to carry out quantitative analysis and structural analysis of the sample sugar chain with high accuracy.
Claims2
A method for analyzing a sugar chain contained in a biological sample by a mass spectrometry method, characterized by including:
- (a) a step of releasing the sugar chain from the biological sample;
- (b) a step of capturing the released sugar chain onto a solid phase carrier;
- (c) a step of re-releasing the captured sugar chain;
- (d) a step of methylating hydroxyl groups of the re-released sugar chain;
- and
- (e) a step of analyzing the methylated sugar chain by the mass spectrometry method.
Dependencies are inferred from the claim text.