T-dna/protein nano-complexes for plant transformation
AssigneePlantbiosis Ltd.
InventorsIgor KOVALCHUK, Alicja ZIEMIENOWICZ, Francois EUDES
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Abstract
A DNA/protein nano-complex for transfecting eukaryotic cells, wherein the nano-complex comprises an expressible nucleotide molecule complexed with a VirD2 protein molecule, and a RecA protein molecule complexed with the nucleotide molecule/VirD2 protein molecule complex. The nucleotide molecule/VirD2 protein molecule/RecA protein molecule complexed may be further complexed to a cell penetrating peptide. The DNA/protein nano-complex can be used for delivering the expressible nucleotide molecule to target plant cells, in particular, monocot plant cells exemplified by wheat cells, corn cells, triticale cells, barley cells, and rye cells.
Claims26
A DNA/protein nano-complex comprising:
- an expressible nucleotide molecule complexed with a VirD2 protein molecule;
- and
- a RecA protein molecule complexed with the nucleotide molecule/VirD2 protein molecule complex.
A method for preparing a DNA/protein nano-complex, comprising the steps of:
- preparing a DNA construct comprising an expressible nucleotide molecule operably linked with a VirD2 target sequence;
- exposing the DNA construct to VirD2 protein molecules whereby the VirD2 target sequence is cleaved and a nucleotide molecule/VirD2 protein molecule complex is formed;
- and
- exposing the nucleotide molecule/VirD2 protein molecule complex to a RecA protein molecule whereby a nucleotide molecule/VirD2 protein molecule/RecA protein molecule complex is formed.
Dependencies are inferred from the claim text.