Method for producing induced pluripotent stem cells and method for culturing the same
AssigneeKyoto University
InventorsShinya Yamanaka, Kazutoshi Takahashi
Life of this patent
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Abstract
[Object] To provide a technology for production of safer iPS cells and to provide a more efficient technology for culturing iPS cells. [Solving Means] Induced pluripotent stem cells are produced from human somatic cells by co-culturing human somatic cells having a reprogrammed nucleus with human cells as feeder cells. Induced pluripotent stem cells are produced from somatic cells by co-culturing somatic cells having a reprogrammed nucleus with autologous cells as feeder cells. Induced pluripotent stem cells are cultured with culture supernatant of somatic cells.
Claims20
A method for producing induced pluripotent stem cells from somatic cells, comprising co-culturing somatic cells having a reprogrammed nucleus with autologous cells as feeder cells.
A method for producing induced pluripotent stem cells from human somatic cells, comprising co-culturing human somatic cells having a reprogrammed nucleus with human cells as feeder cells.
A method for culturing induced pluripotent stem cells, comprising co-culturing induced pluripotent stem cells with human somatic cells as feeder cells.
A method for culturing induced pluripotent stem cells, comprising culturing induced pluripotent stem cells with culture supernatant of somatic cells.
A culture medium for culturing induced pluripotent stem cells, wherein said culture medium comprises culture supernatant of human somatic cells.
Dependencies are inferred from the claim text.