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US 2012/0070890 A1Published application
Targeted gene modification by parvoviral vectors
AssigneeUniversity of Washington
InventorsDavid W. Russell, Roli K. Hirata
Life of this patent
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Abstract
This invention provides methods for obtaining targeted gene modification in vertebrate cells using parvoviral vectors, including adeno-associated virus (AAV). The parvoviral vectors used in the methods of the invention are capable of targeting a specific genetic modification to a preselected target locus in a cellular genome by homologous pairing.
Claims25
(canceled)
A recombinant parvoviral vector for introducing a genetic modification at a preselected genomic target locus of a vertebrate cell, the recombinant parvoviral vector comprising:
- a targeting construct which comprises a genomic DNA sequence which is substantially identical to the genomic target locus except for the modification to be introduced, wherein the modification to be introduced is flanked by regions substantially identical to the genomic target locus, said regions being at least 100 nucleotides in length;
- and
- at least one parvoviral inverted terminal repeat (ITR) flanking the targeting construct.
Dependencies are inferred from the claim text.