Reduction of antibiotic activity or concentration in biological samples using molecularly imprinted polymers
AssigneeBecton, Dickinson and Company
InventorRobert Sallitt
Life of this patent
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Abstract
Methods of removing undesired compounds such as antibiotics from a biological sample are provided. The methods use Molecularly Imprinted Polymers (“MIPs”) to remove or inactivate antibiotics in a sample. The methods use MIPS to remove or inactivate compounds such as antibiotics in a cell culture.
Claims47
A method of reducing the activity of an antibiotic in a sample comprising:
- adding a Molecularly Imprinted Polymer (MIP) that is configured to bind to the antibiotic in the sample, wherein the MIP binds the antibiotic in an amount effective to reduce the activity of the antibiotic in the sample.
A method of reducing the activity of a class of antibiotics in a sample comprising:
- adding a Molecularly Imprinted Polymer (MIP) that is configured from a template to bind at least two antibiotics selected from the class of antibiotics in the sample, wherein the MIP binds to the at least two antibiotics in the class of antibiotics in an amount effective to reduce the activity of the at least one antibiotic in the sample.
A method of reducing the concentration of an antibiotic in a fluid sample comprising:
- collecting fluid sample with a first concentration of antibiotic in a collection device, wherein the collection device has Molecularly Imprinted Polymers (MIPs) disposed on at least a portion of a surface of the collection device that contacts the fluid sample, and wherein the MIPs are configured to bind the antibiotic;
- contacting the fluid sample with the MIPs, thereby allowing the antibiotic to bind to the MIPs attached to the collection device;
- and
- removing the fluid sample from the collection device, wherein the concentration of the antibiotic in the removed portion of the fluid sample is a second concentration that is less than the first concentration.
A method of reducing the activity of an antibiotic in a culture comprising:
- inoculating a cell culture with bacterial cells, wherein the cell culture comprises media that further comprises Molecularly Imprinted Polymers (MIPs) that are configured to bind to an antibiotic that may be present in the culture;
- and
- contacting the MIPs with the antibiotic, if present, thereby allowing the MIPs to specifically bind to the antibiotic, thereby reducing the activity of the antibiotic in the cell culture.
A method of reducing the concentration of an antibiotic in a fluid sample comprising:
- adding Molecularly Imprinted Polymers (MIPs) attached to beads to the fluid sample, wherein the MIPs attached to the beads are configured to bind to at least one antibiotic;
- contacting the MIPs with the fluid sample, thereby allowing the at least one antibiotic to bind to the MIPs attached to beads;
- and
- separating the beads from the fluid sample, thereby removing at least a portion of the antibiotic from the fluid sample and reducing the concentration of the antibiotic in the fluid sample.
A method of reducing the concentration of at least one antibiotic from a fluid sample comprising:
- collecting the fluid sample in a collection device, wherein Molecularly Imprinted Polymers (MIPs) are contained within the collection device;
- contacting the fluid sample in the collection device with the MIPs, wherein the fluid sample has a first concentration of antibiotics in the sample and wherein the MIPs are configured to bind the at least one antibiotic in a class of antibiotics;
- allowing the at least one antibiotic in the class of antibiotics to bind to the MIPs contained within the collection device;
- and
- removing the fluid sample from the collection device, wherein the removed fluid sample has a second concentration of at least one antibiotic in the fluid sample that is less than the first concentration of the antibiotic in the fluid sample.
A kit comprising media for culturing cells, wherein the media comprises at least one MIP configured to bind to at least one antibiotic.
A kit comprising media for culturing cells, wherein the media comprises at least one MIP configured to bind to a class of antibiotics.
A collection device for the collection of a fluid sample, wherein at least one MIP configured to bind to at least one antibiotic is attached to at least one surface of the collection device, and wherein the at least one surface of the collection device is configured to contact fluid sample introduced into the collection device.
Dependencies are inferred from the claim text.