Method for recycling enzyme
AssigneeJapan International Research Center for Agricultural Sciences
InventorsAkihiko Kosugi, Rattiya Waeonukul, Yutaka Mori
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Abstract
In saccharification of cellulose, chimeric β-glucosidase having a region exhibiting thermophilic bacteria-derived β-glucosidase activity and a module combinable to cellulose is used along with cellulosome, and at the completion of saccharification of cellulose, a cellulosic substrate is added to make the chimeric β-glucosidase and cellulosome attach to the cellulosic substrate for separation.
Claims21
A method for recycling an enzyme, comprising:
- combining an enzyme having a module combinable with cellulose and a cellulosic substrate, and
- using the cellulosic substrate-bound enzyme obtained repeatedly.
The method for recycling an enzyme as set forth in 7, wherein the enzyme having a module combinable with cellulose is reacted with the cellulosic substrate in the absence of a reducing agent.
The method for recycling an enzyme as set forth in 8, wherein the enzyme having a module combinable with cellulose is reacted with the cellulosic substrate in the absence of a reducing agent.
The method for recycling an enzyme as set forth in 9, wherein the enzyme having a module combinable with cellulose is reacted with the cellulosic substrate in the absence of a reducing agent.
The method for recycling an enzyme as set forth in 12, wherein the enzyme having a module combinable with cellulose is reacted with the cellulosic substrate in the absence of a reducing agent.
β-glucosidase containing a region that exhibits β-glucosidase activity derived from thermophilic bacteria and a module combinable with cellulose derived from cellulosome generated by Clostridium.
Dependencies are inferred from the claim text.