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AssigneeToray Industries, Inc.
InventorsKenji Sawai, Kazumi Suda, Hideki Sawai, Katsushige Yamada, Junya Yamagishi
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Highly productive D-lactic acid fermentation uses a transformant obtained by introducing into a host cell a polynucleotide encoding a polypeptide according to any one of the following (A) to (C) in such a manner that the polypeptide is expressed, which polypeptide has a D-lactate dehydrogenase activity higher than those of conventional polypeptides: (A) a polypeptide having the amino acid sequence shown in SEQ ID NO:1 or 2; (B) a polypeptide having the same amino acid sequence as shown in SEQ ID NO:1 or 2 except that one or several amino acids are substituted, deleted, inserted and/or added, which polypeptide has a D-lactate dehydrogenase activity; and (C) a polypeptide having an amino acid sequence which has a sequence identity of not less than 80% to the amino acid sequence shown in SEQ ID NO:1 or 2, which polypeptide has a D-lactate dehydrogenase activity.
A polypeptide comprising any one of the following (A) to (C):
A transformant in which a polynucleotide encoding D-lactate dehydrogenase originated from the family Limulidae or a DNA construct in which said polynucleotide and a promoter capable of expressing said polynucleotide are linked is introduced.
A transformed yeast in which a polynucleotide encoding D-lactate dehydrogenase originated from the family Limulidae or a DNA construct in which said polynucleotide and a promoter capable of expressing said polynucleotide are linked is introduced.
Dependencies are inferred from the claim text.