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AssigneeRegeneron Pharmaceuticals, Inc.
InventorsLynn Macdonald, Sean Stevens, Cagan Gurer, Andrew J. Murphy, Karolina A. Hosiawa
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Genetically modified mice are provided that express human λ variable (hVλ) sequences, including mice that express hVλ sequences from an endogenous mouse λ light chain locus, mice that express hVλ sequences from an endogenous mouse κ light chain locus, and mice that express hVλ sequences from a transgene or an episome wherein the hVλ sequence is linked to a mouse constant sequence. Mice are provided that are a source of somatically mutated human λ variable sequences useful for making antigen-binding proteins. Compositions and methods for making antigen-binding proteins that comprise human λ variable sequences, including human antibodies, are provided.
A mouse comprising in its germline, at an endogenous mouse light chain locus, a human λ light chain variable region sequence, wherein the human λ variable region sequence is expressed in a light chain that comprises a mouse immunoglobulin constant region gene sequence.
A mouse that expresses an immunoglobulin light chain that comprises a human lambda variable sequence fused with a mouse constant region, wherein the mouse exhibits a κ usage to λ usage ratio of about 1:1.
A method for making a human λ variable region nucleic acid sequence, comprising a step of cloning from a cell a human λ variable region nucleic acid sequence, wherein the cell is derived from a mouse that comprises in its germline a hVλ gene segment and a hJλ gene segment operably linked to a mouse constant region nucleic acid sequence at an endogenous mouse constant light chain locus.
Dependencies are inferred from the claim text.