Bacterial cells, optimized nucleotide sequences and methods for improved expression of recombinant clostridium difficile toxin b

An isolated nucleotide sequence encoding Clostridium difficile toxin B,

• wherein said isolated nucleotide sequence has been optimized for improved expression of recombinant toxin B in a bacterial cell,
• wherein said optimization comprises the identification of one or more codons that rarely appear in said bacterial cell, and the replacement of said one or more codons with one or more codons that more frequently appear in said bacterial cell.

A bacterial cell comprising an isolated nucleotide sequence encoding Clostridium difficile toxin B,

• wherein said isolated nucleotide sequence has been optimized for improved expression of toxin B in said bacterial cell;
• and
• wherein said isolated nucleotide sequence comprises at least one of SEQ ID NOS.1, 3, 5, 6, 7, 8, 9, 10 and 11

A bacterial cell comprising an isolated nucleotide sequence encoding Clostridium difficile toxin B,

• wherein said isolated nucleotide sequence has been optimized for improved expression of toxin B in said bacterial cell,
• wherein said optimization comprises the identification of one or more codons that rarely appear in said bacterial cell, and the replacement of said one or more codons with one or more codons that more frequently appear in said bacterial cell.

An isolated peptide of recombinant Clostridium difficile toxin B, wherein said isolated peptide sequence comprises at least one of SEQ ID NOS.12-17.