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OwnerLionel Fernel Gamarra Contreras, Daiane Dona Guilhen +3inventors
InventorsLionel Fernel Gamarra Contreras, Daiane Dona Guilhen, Mariano Janiszewski, Lonena Favaro Pavon, Luciana Cavalheiro Marti
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A method for isolating exosomes from blood platelets using superparamagnetic nanoparticles of iron oxide (Fe3O4), by means of a charge attraction mechanism based on the predetermined Zeta potential of the exosomes. The method involves the use of iron oxide nanoparticles that are previously synthesised with a predetermined positive charge, and that bond to the negatively charged exosomes contained in the biological sample. During incubation, the cationic magnetic nanoparticles are absorbed by the surface of the membrane of the exosomes owing to electrostatic interaction. Exposure of the material to a magnetic field makes it possible to separate the exosomes bonded to the nanoparticles. The success of this technique has been confirmed by characterisation of the exosomes by flow citometry. The method has been shown to be suitable for this purpose, since it allows exosomes to be isolated and purified, without undergoing alterations of their original morphological and structural characteristics.
A method for isolation of exosomes from biological solutions using nanoparticles of iron oxide, featured by the fact that the method for exosomes isolation comprise platelets using superparamagnetic magnetite nanoparticles (Fe3O4), and be made through an attraction charge mechanism based on the exosomes pre-determined zeta potential where the iron oxide nanoparticles where previously synthetized with pre-determined positive charge, linked to the exosomes negatively charged, comprised in the biological sample, by means of electric attraction;
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