Method for in vitro assay of soluble fibrin by generating specific degradation products
AssigneesAssistance Publique-Hopitaux de Paris, Societe Diagnostica-Stago
InventorsBibi Shah Soltan Mirshahi, Jeannette Soria
Life of this patent
Estimating the term…
Abstract
The invention concerns a method for assaying soluble fibrin in a sample, in which said sample is brought into the presence of a plasminogen activator with a high specificity for soluble fibrin (PA-Fb sp) and the soluble fibrin count in the sample is measured by measuring the difference between the count of fibrin degradation products obtained after degrading soluble fibrin with PA-Fb sp and the base count of fibrin degradation products determined before bringing the sample into the presence of PA-Fb sp.
Claims12
A kit for assaying soluble fibrin in a sample, comprising:
- PA-Fb sp in individual quantities for a single sample or in a quantity sufficient for multiple samples;
- and
- a reagent to assay soluble fibrin degradation products.
An assay method comprising the steps of:
- contacting a sample with a PA-Fb sp to generate an amount of fibrin degradation products;
- measuring said amount of fibrin degradation products;
- and
- comparing the amount of fibrin degradation products with the amount of fibrin degradation products of either a sample provided without contact with PA-FB sp or a positive control.
A method for assaying soluble fibrin in a sample, comprising:
- measuring a difference in an amount of fibrin degradation products between a sample including a plasminogen activator with a high specificity and/or affinity for soluble fibrin (PA-Fb sp) and a sample excluding said PA-FB sp and wherein said PA-Fb sp is SAK in a final concentration of no more than about 12 μg/ml of said sample.
Dependencies are inferred from the claim text.