Assay
AssigneeImperial Innovations Limited
InventorsMolly Stevens, James Eric Ghadiali
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Abstract
The present invention relates to an assay for transferases. The assay comprises a first moiety comprising a transferase substrate and a second moiety capable of binding to the transferase substrate after it has been acted on by the transferase. One of the first and second moieties comprises a fluorophore and the other of the first and second moieties causes a change in fluorescence of the fluorophore. Thus, when the second moiety binds the transferase substrate after it has been acted on by the transferase, a change in fluorescence can be detected. The assay allows for agents that modulate the activity of the transferase to be screened.
Claims22
An assay, comprising:
- a first moiety comprising a transferase substrate;
- and
- a second moiety capable of binding to the transferase substrate after it has been acted on by the transferase,
- wherein one of the first and second moieties comprises a fluorophore and the other of the first and second moieties causes a change in fluorescence of the fluorophore, such that, when the second moiety binds the transferase substrate after it has been acted on by the transferase, a change in fluorescence can be detected.
A method for screening for an agent that modulates the activity of a transferase, comprising:
- contacting the transferase, in the presence of an agent to be screened, with a first moiety comprising a transferase substrate;
- and a second moiety capable of binding to the transferase substrate after it has been acted on by the transferase,
- wherein one of the first and second moieties comprises a fluorophore and the other of the first and second moieties causes a change in fluorescence of the fluorophore, such that, when the second moiety binds the transferase substrate after it has been acted on by the transferase, a change in fluorescence can be detected, and wherein the agent is a modulator of the activity of the transferase if the change in fluorescence is altered in the presence of the agent compared to the change in fluorescence when the agent is absent.
Dependencies are inferred from the claim text.