Method for detecting microorganisms and microorganism detecting apparatus
AssigneeNihon Dempa Kogyo Co., Ltd.
InventorsMitsuaki Koyama, Shunichi Wakamatsu, Wakako Shinobu
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Abstract
It is possible to determine the presence of bacteria in a sample solution in a shorter period of time without changing a conventional incubating method. Bacteria in a sample solution are incubated in, for example, a sterilized agar medium 10 having a layer thickness of 0.1 μm to 1 μm formed on an electrode of a crystal resonator 2, and an oscillation frequency is measured. When the bacteria proliferate, the mass of the entire crystal resonator 2 increases, and the oscillation frequency decreases. Therefore, by monitoring presence of such a change over time, presence of bacteria in the sample solution can be determined quickly.
Claims12
A method for detecting microorganisms, comprising:
- using a piezoelectric vibrator having electrodes formed on both faces and supplying a culture medium layer, which is an absorption layer formed on the electrode on one face side of the piezoelectric vibrator, with a sample solution in which it is possible that microorganisms which are detection targets are mixed;
- and oscillating the piezoelectric vibrator by an oscillation circuit and measuring an oscillation frequency of the piezoelectric vibrator, wherein at least one of presence of microorganisms and a rate of proliferation of microorganisms is obtained based on a change over time in the oscillation frequency.
A method for detecting microorganisms, comprising:
- using a piezoelectric vibrator having electrodes formed on both faces and supplying an antibody layer, which is an absorption layer formed on the electrode on one face side of the piezoelectric vibrator, with a sample solution in which it is possible that microorganisms which are detection targets having an antigen to be absorbed in the antibody layer through antigen-antibody reaction are mixed;
- supplying the antibody layer with a liquid culture medium;
- and oscillating the piezoelectric vibrator by an oscillation circuit and measuring an oscillation frequency of the piezoelectric vibrator, wherein at least one of presence of microorganisms and a rate of proliferation of microorganisms is obtained based on a change over time in the oscillation frequency.
-15. (canceled)
Dependencies are inferred from the claim text.