Methods of detecting residual amounts of polymers used in the purification of biomolecules
AssigneeMillipore Corporation
InventorsMartin Zillmann, Jad Jaber, James Hamzik
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Abstract
The present invention relates to methods of detecting residual amounts of polymers including stimulus responsive polymers, which are used in processes for purifying biomolecules such as, for example, proteins, polypeptides, antibodies, vaccines and the like.
Claims47
A method of detecting residual amounts of a polymer in a sample comprising a biomolecule of interest, wherein the polymer is used for separating the biomolecule of interest from one or more impurities, the method comprising the steps of:
- (1) contacting the sample with the polymer, wherein the polymer is associated with a tag;
- and (2) detecting the tag,
- wherein the amount of tag detected is indicative of the amount of residual polymer in the solution comprising the biomolecule of interest.
A method for detecting residual amounts of a stimulus responsive polymer used for separating a biomolecule of interest from one or more impurities, the method comprising the steps of:
- (1) contacting a solution containing a biomolecule of interest and one more impurities with a stimulus responsive polymer, where the polymer is associated with a tag, such that to form a complex of polymer and one or more impurities;
- (2) applying a stimulus to the solution, thereby to precipitate the complex;
- (3) removing the precipitate from the solution;
- and
- (4) detecting the tag in the solution containing the biomolecule of interest, where the amount of tag detected is indicative of the amount of residual polymer in the solution comprising the biomolecule of interest.
A method for detecting residual amounts of a stimulus responsive polymer used for separating a biomolecule of interest from one or more impurities in a solution, the method comprising the steps of:
- (1) contacting a solution containing a biomolecule of interest and one or more impurities with a stimulus responsive polymer, where the polymer is associated with a tag and where the polymer forms complexes with both the biomolecule of interest as well as the one or more impurities under a first set of conditions;
- (2) adding a stimulus to the solution, thereby to precipitate the complexes;
- (3) subjecting the precipitate to a second set of conditions, thereby to selectively elute the biomolecule of interest from the complex;
- and
- (4) detecting the tag in the eluate containing the biomolecule of interest.
- wherein the amount of tag detected is indicative of the amount of residual polymer in the eluate.
Dependencies are inferred from the claim text.