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US 2012/0070835 A1Published application
Method of Cloning Stable Stress Tolerant Superoxide Dismutase Using Universal Primers
AssigneeCouncil of Scientific and Industrial Research
InventorsPardeep Kumar Bhardwaj, Arun Kumar, Amit Kishore, Sanjay Ghawana, Arti Rani, Kashmir Singh, Harsharan Singh, Ravi Shankar Singh, Hitesh Kumar, Payal Sood, Som Dutt, Sanjay Kumar, Paramvir Singh Ahuja
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Abstract
The present invention relates to a method of cloning stable stress tolerant superoxide dismutase from diverse plant species using universal primers.
Claims21
Set of degenerate primer useful for amplifying stress tolerant superoxide dismutase from diverse plant species wherein the said set comprises of:
- i. SEQ ID No.[[22]] 24 & 25.
- Forward primer =
- (SEQ ID No. 24)
- 5′CAGGAAGGAGATGG(C/T)CCAAC(A/C) 3′
- Reverse primer =
- (SEQ ID No. 25)
- 5′ (C/T)TGAA(A/G)(A/G)CC(A/G)AT(G/A/C)CCACAAGC 3′
- ii. SEQ ID No. [[23]] 26 & 27.
- Forward primer =
- (SEQ ID No. 26)
- 5′ TC(A/T)AC(C/T)GG(G/A/T)CC(A/G)CA(C/T)TA(C/T)
- AAT 3′
- Reverse primer =
- (SEQ ID No. 27)
- 5′(C/T)TGAA(A/G)(A/G)CC(A/G)AT(G/A/C)CCACAAGC 3′
- wherein the said primers are used alone or in combination.
13 more elements
Dependencies are inferred from the claim text.