Se33 mutations impacting genotype concordance

A method for amplifying a nucleic acid comprising:

• a. providing at least one forward primer capable of annealing to SE33 locus 5′ of a STR region,
• b. providing at least a first reverse primer capable of annealing to the SE33 locus 3′ of the STR region, wherein said reverse primer anneals to at least 4 contiguous nucleotides from nucleotide positions 282 to 332 and/or nucleotide positions 351 to 359 as illustrated in SEQ ID NO:3.

An amplification primer set for SE33 locus comprising:

• a. a first primer capable of annealing to a primer-binding site 5′ of SE33's STR region;
• and
• b. a second primer, a reverse primer, capable of annealing to a first primer-binding site 3′ of the SE33 STR region, wherein the first primer-binding site comprises one variant SNP, wherein the variant SNP is selected from one of nucleotide positions 316, 317 and 324 as illustrated in SEQ ID NO:3.

A kit for analyzing a plurality of STR loci in a nucleic acid sample, wherein the kit comprises at least a first SE33 STR locus primer set, wherein the SE33 primer set generates a first amplification product for said nucleic acid sample comprising a SNP at one of positions 316, 317 or 324 as illustrated in SEQ ID NO:3.