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US 2012/0070829 A1Published application
Size selection of dna for chromatin analysis
AssigneeBio-Rad Laboratories, Inc.
InventorsSteven Okino, Yan Wang
Life of this patent
Estimating the term…
Abstract
Methods for analyzing chromosomal DNA, including chromatin, are provided.
Claims25
A method for analyzing chromosomal DNA, the method comprising,
- a. introducing a DNA cleaving agent into a cell having genomic DNA under conditions such that the DNA cleaving agent cleaves the genomic DNA in the cell, wherein different regions of the genomic DNA are cleaved to a different extent by the agent, thereby generating cleaved and intact DNA regions;
- and
- b. enriching the DNA for either intact or cleaved DNA, thereby generating enriched DNA;
- and
- c. detecting a presence, absence, or quantity of intact or cleaved copies of at least one DNA region in the enriched DNA or cloning, isolating, or nucleotide sequencing at least one intact or cleaved DNA region in the enriched DNA.
2 more elements
A method for analyzing chromosomal DNA, the method comprising,
- a. introducing a DNA modifying agent into a cell having genomic DNA under conditions such that the DNA modifying agent modifies the genomic DNA in the cell, wherein different regions of the genomic DNA are modified to a different extent by the agent, thereby generating modified and unmodified DNA regions, and subsequently
- b. contacting the genomic DNA in, or isolated from, the cell with a DNA cleaving agent, wherein different regions of the genomic DNA are cleaved to a different extent by the DNA cleaving agent, thereby generating cleaved and intact DNA regions;
- and
- c. enriching the DNA for either intact or cleaved DNA, thereby generating enriched DNA;
- and
- d. detecting a presence, absence, or quantity of intact or cleaved copies of at least one DNA region in the enriched DNA or cloning, isolating, or nucleotide sequencing at least one intact or cleaved DNA region in the enriched DNA.
3 more elements
Dependencies are inferred from the claim text.