Method for signal amplification during lateral-flow analysis
OwnerByeong-Woo Bae, Sung-Dong Lee +5inventors
InventorsByeong-Woo Bae, Sung-Dong Lee, Min-Gon Kim, Youn-Beom Shin, Jin-Hee Jang, Ji-Hun Shin, Seok-Ki Lee
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Abstract
Provided is a signal amplifying method in a lateral flow analysis with high sensitivity, in which gold ions and a reductant are added and react to a seed of gold nano particles to amplify a signal, and a lateral flow analysis device using the same.
Claims14
A signal amplifying method in a lateral flow analysis, the method comprising:
- combining a conjugate with an analyte, the conjugate including a first antibody or a first specific combining material, which is specifically combined with a first epitope or a first combining portion (ligand) of the analyte, and gold nano particles having the first antibody or the first specific combining material combined;
- combining the analyte having the combined conjugate with a fixed second antibody or second specific combining material, which is specifically combined with a second epitope or a second combining portion (ligand) of the analyte;
- and
- adding gold ions and a reductant to cause reaction.
A lateral flow analysis device comprising:
- a bonding pad including a conjugate having a first antibody or a first specific combining material, which is specifically combined with a first epitope or a first combining portion (ligand) of an analyte, and gold nano particles having the first antibody or the first specific combining material combined;
- a membrane having a detection site including a fixed second antibody or second specific combining material, which is specifically combined with a second epitope or a second combining portion of the analyte having the conjugate combined;
- and
- a pad for signal amplification including gold ions and a reductant.
A lateral flow analysis device set comprising a lateral flow analysis device and an additional container containing a solution for signal amplification, wherein the solution for signal amplification includes gold ions and a reductant.
A biometric data analysis system comprising:
- a measuring device including a lateral flow analysis device and a reading unit for reading test results of the lateral flow analysis device,
- wherein the measuring device includes:
- a storage unit for storing a solution for signal amplification;
- and
- a unit for applying the solution for signal amplification to the lateral flow analysis device, wherein the solution for signal amplification includes gold ions and a reductant.
Dependencies are inferred from the claim text.