Mitochondrial inhibitors and uses thereof
AssigneeUniversity of Miami
InventorsTheodore Lampidis, Metin Kurtoglu
Life of this patent
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Abstract
Compositions which modulate mitochondrial functions treat diseases associated with cells that are hyperactively using their endoplasmic reticulum. Screening assays identify agents which modulate mitochondrial functions.
Claims43
A method of identifying candidate therapeutic agents for treatment of diseases or disorders comprising:
- culturing a biological sample with at least one candidate therapeutic agent;
- measuring levels of calcium ions (Ca2+) in the biological samples mitochondrial and endoplasmic reticulum in the presence or absence of a candidate therapeutic agent as compared to controls;
- comparing the levels of calcium ions (Ca2+) at one or more time points;
- and,
- identifying candidate therapeutic agents for treatment of diseases or disorders.
A method of identifying a modulator of mitochondrial Ca2+ uptake activity comprising:
- contacting a cell with a candidate agent;
- measuring exchange of calcium ion (Ca2+) between mitochondria and ER in the presence or absence of a candidate therapeutic agent as compared to controls;
- and,
- identifying a modulator of mitochondrial Ca2+ uptake activity.
A composition for modulating mitochondrial function comprising at least one peroxisome proliferator-activated receptor (PPAR) agonist.
A composition for modulating mitochondrial function comprising a peroxisome proliferator-activated receptor (PPAR) agonist and a mitochondrial calcium blocker.
A composition for inducing endoplasmic reticulum hyperactivity comprising a glucose metabolic inhibitor.
A method of treating a disease associated with high endoplasmic reticulum function comprising:
- contacting a cell in vitro or in vivo with an agent which modulates mitochondrial function.
A method of treating a disease associated with high levels of mitochondrial calcium ions and/or low levels of endoplasmic reticulum calcium ions comprising treating a patient with an agent which inhibits mitochondrial function.
- exchange of calcium ion (Ca2+) between mitochondria and ER in the presence or absence of a candidate therapeutic agent as compared to controls;
- and,
- identifying a modulator of mitochondrial Ca2+ uptake activity.
A method of treating a patient with an abnormal cell disorder comprising administering to the patient an inhibitor of mitochondrial function.
Dependencies are inferred from the claim text.