Antibodies containing therapeutic tpo/epo mimetic peptides
AssigneeAlexion Pharmaceuticals, Inc.
InventorsJeremy P. Springhorn, David Gies
Life of this patent
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Abstract
The present disclosure features therapeutic antibodies (e.g., TPO mimetic antibodies) and therapeutically-active fragments thereof as well as methods for preparing and using the antibodies and fragments. For example, the therapeutic antibodies and their fragments are useful in a variety of diagnostic and/or therapeutic applications such as methods for increasing platelet levels in a subject.
Claims36
A therapeutic antibody, or a therapeutically-active fragment of the antibody, wherein the antibody or fragment comprises at least two therapeutic peptides and wherein at least one of the therapeutic peptides is incorporated into the constant region of a light chain polypeptide of the antibody or fragment at a position that is structurally permissive to allow for the therapeutic peptide to be presented at the central cleft of the antibody.
A therapeutic antibody, or a therapeutically-active fragment of the antibody, wherein the antibody or fragment comprises at least two therapeutic peptides, wherein a heavy chain polypeptide of the antibody or fragment comprises at least one of the therapeutic peptides, and wherein the at least one therapeutic peptide is incorporated:
- (a) into the hinge region of the heavy chain polypeptide;
- (b) at the junction between the amino-terminus of the hinge region and the region of the heavy chain polypeptide that is upstream to the hinge region;
- (c) at the junction between the carboxy-terminus of the hinge region and the region of the heavy chain polypeptide that is downstream of the hinge region;
- or
- (d) at a location starting within fewer than 20 amino acids upstream of the amino-terminus, or fewer than 20 amino acids downstream of the carboxy-terminus, of the hinge region of the heavy chain polypeptide.
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An isolated polypeptide comprising an amino acid sequence that comprises at least one TPO mimetic peptide amino acid sequence and is:
- (i) at least 80% identical to the amino acid sequence depicted in SEQ ID NO:10;
- or
- (ii) at least 80% identical to the amino acid sequence depicted in SEQ ID NO:12.
An isolated nucleic acid encoding a polypeptide comprising an amino acid sequence that comprises at least one TPO mimetic peptide amino acid sequence, wherein said polypeptide is:
- (i) at least 80% identical to the amino acid sequence depicted in SEQ ID NO:10;
- or
- (ii) at least 80% identical to the amino acid sequence depicted in SEQ ID NO:12.
Dependencies are inferred from the claim text.