Flavonoid hydrogel
AssigneeAgency for Science, Technology and Research
InventorsMotoichi Kurisawa, Fan Lee, Joo Eun Chung, Pui Yik Chan
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Abstract
There is provided methods for producing a hydrogel comprising conjugates of a hydrogel forming agent and a flavonoid including a method for producing a hydrogel that is capable of adhesion of cells and which comprises enzymatically cross-linked conjugates of a hydrogel forming agent and a flavonoid. There is also provided a method for producing a hydrogel comprising conjugates of a hydrogel forming agent and a flavonoid without the addition of an exogenous peroxide or peroxidase or without the addition of an exogenous peroxide. Hydrogels produced by such methods and methods of using the hydrogels are also described herein.
Claims45
A method for producing a hydrogel that is capable of adhesion of cells and which comprises enzymatically cross-linked conjugates of a hydrogel forming agent and a flavonoid, the method comprising combining:
- (i) from about 0.1 mg/ml to about 500 mg/ml of conjugates of the hydrogel forming agent and the flavonoid;
- (ii) from about 0.001 mM to about 50 mM peroxide;
- and
- (iii) from about 0.001 units/ml to about 10 units/ml peroxidase;
- thereby producing the hydrogel.
A hydrogel capable of adhesion of cells and which comprises enzymatically cross-linked conjugates of a hydrogel forming agent and a flavonoid, the hydrogel produced by a method comprising combining:
- (i) from about 0.1 mg/ml to about 500 mg/ml of conjugates of the hydrogel forming agent and the flavonoid;
- (ii) from about 0.001 mM to about 50 mM peroxide;
- and
- (iii) from about 0.001 units/ml to about 10 units/ml peroxidase.
A method for producing a hydrogel comprising conjugates of a hydrogel forming agent and a flavonoid, the method comprising combining the conjugates in a solution in the absence of an exogenously added peroxide and in the absence of a peroxidase.
A method for producing a hydrogel comprising conjugates of a hydrogel forming agent and a flavonoid, the method comprising combining the conjugates and a peroxidase in a solution in the absence of an exogenously added peroxide.
Dependencies are inferred from the claim text.