Method of altering the differentiative state of a cell and compositions thereof
AssigneeInternational Stem Cell Corporation
InventorTrudy Christiansen-Weber
Life of this patent
Estimating the term…
Abstract
The present invention provides a method of altering the differentiative state of cells utilizing innovative protein expression constructs encoding transcription factors. The methods and compositions described herein may be used to generate induced pluripotent stem (iPS) cells, as well as differentiate, transdifferentiate or dedifferentiate cells of various epigenetic status. The method includes introduction of a nucleic acid construct, or expression product thereof, into a cell, and culture of the cell under culture conditions that efficiently converts the cell into a pluripotent cell, enhances the retention of the pluripotent state or efficiently converts the cell into a cell of a cell lineage corresponding to endoderm, mesoderm or ectoderm.
Claims53
A method of reprogramming, differentiating, transdifferentiating or dedifferentiating a target or recipient cell into a cell of a different cell type or into a pluripotent cell or less differentiated cell by introducing one or more nucleic acid constructs into the cell, or the expression product thereof, and culture under culture conditions that convert the cell into a pluripotent cell or into a cell of a cell lineage corresponding to endoderm, mesoderm or ectoderm.
A nucleic acid construct encoding a cassette comprising in operable linkage:
- i) at least one protein tag;
- ii) a protein transduction domain;
- iii) a fusion domain;
- iv) a nuclear localization signal;
- and
- v) a transcription factor.
Dependencies are inferred from the claim text.