Yeast cell wall particles for receptor-targeted nanoparticle delivery
AssigneeUniversity of Massachusetts
InventorsGary R. OSTROFF, Ernesto SOTO
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Abstract
The present invention generally relates to yeast cell wall microparticles loaded with nanoparticles for receptor-targeted nanoparticle delivery. In particular, the present invention relates to trapping nanoparticles either on the surface or inside a yeast glucan particles, for example, yeast glucal particles. The present invention further relates to methods of making the yeast cell wall particles loaded with nanoparticles. The present invention also relates to methods of using the yeast cell wall particles loaded with nanoparticles for receptor-targeted delivery of the nanoparticles, e.g., drug containing nanoparticles.
Claims32
A yeast cell wall particle-nanoparticle (YCWP-NP) delivery system, comprising yeast cell wall particles (YCWPs) comprising nanoparticles (NPs) having at least one dimension of about 1-40 nm contained within the interior of the YCWPs, wherein the YCWP-NP delivery system is prepared by a process comprising the steps of:
- (a) loading the NPs into the YCWPs, wherein the loading comprises incubating a suspension comprising YCWPs and NPs for a time sufficient for the NPs to enter into the YCWPs,
- (b) drying the material resulting from (a),
- (c) optionally repeating steps (a)-(b) at least once,
- (d) resuspending the YCWPs and nanoparticles in solvent for a time sufficient for additional NPs to enter into the YCWPs,
- (e) optionally drying the material resulting from (d), and
- (f) optionally trapping the NPs in the YCWPs using an appropriate trapping means,
- such that the YCWP-NP delivery system is prepared.
A yeast cell wall particle-nanoparticle (YCWP-NP) delivery system, comprising yeast cell wall particles (YCWPs) comprising nanoparticles (NPs) having at least one dimension of about 10-1000 nm on the surface of the YCWPs, wherein the YCWP delivery system is prepared by a process comprising the steps of:
- (a) loading the NPs onto the YCWPs, wherein the loading comprises incubating a suspension comprising YCWPs and NPs for a time sufficient for the NPs to bind to the YCWPs, wherein the YCWPs are surface-derivitized such that NP binding is facilitated,
- (b) washing and/or dispersing the material resulting from (a) and
- (c) optionally repeating steps (a)-(b) at least once,
- such that the YCWP-NP delivery system is prepared.
The YCWP-NP delivery system of claim 29, wherein the trapping polymer is selected from the group consisting of polyethyleneamine (PEI), polylysine (PLL) and chitosan.
A method of using the YCWP-NP delivery system of any one of the preceding claims in drug delivery.
A method of using the YCWP-NP delivery system of any one of the preceding claims in medical imaging.
A method for making the YCWP-NP delivery system of any one of the preceding claims comprising the steps recited in said claims.
Dependencies are inferred from the claim text.