US 2012/0067742 A1 · LINKER, IMPEDANCE BIOCHIP, AND METHOD OF QUANTITATIVELY DETECTING TARGET ANALYTE IN FLUID SAMPLE USING THE BIOCHIP

Abstract

The present invention provides a linker for joining an electrode and a capture probe on a biochip, and a biochip comprising the linker. The impedance baseline of the linker of the present invention is three orders lower than the conventional long chain thiol linker when adopting in a fadaraic impedance biochip construction. With lower impedance baseline, the device designed to measure the signal of the biochip of the present invention could be further simplied on the electrical circuit design and be made in lower cost, compacter size and get the potential to be used in point-of-care applications. The present invention also provides a method of quantitatively detecting a concentration of a target analyte in a fluid sample by adopting the biochip and the linker of present invention.

Claims17

Dependent claims

A linker for joining an electrode and a capture probe on a biochip, comprising a compound of formula (I):

R2—(CH2)m—(R3)n—(CH2)k—R1 (I)
wherein
R1 is a functional group for binding the capture probe which is protein, DNA, RNA, or enzyme;
R2 is a functional group for connecting the electrode selected from the groups consisting of Au, Pt, Ag, and ITO;
R3 is a thiophene or thiophene derivative;
n is an integer of from 1 to 4;
m is an integer of from 0 to 5;
and
k is an integer of from 0 to 5.

6 more elements

Dependencies are inferred from the claim text.

Facts

Application

13/161,702Filed 16 Jun 2011

Priority

17 Sep 2010

Published

22 Mar 2012

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

C07D 333/34
Hetero atoms other than halogen: sulfur atoms
C07D 333/18
Radicals substituted by singly bound hetero atoms other than halogen: by sulfur atoms
C07D 333/22
With only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to the ring carbon atoms: radicals substituted by…
C07D 333/38
With hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals,…:…

Linker, impedance biochip, and method of quantitatively detecting target analyte in fluid sample using the biochip

Life of this patent

Linker, impedance biochip, and method of quantitatively detecting target analyte in fluid sample using the biochip

Life of this patent

Abstract

Claims17