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US 2012/0060237 A1Published application
Method for enhancing thermotolerance of plant and genetic engineering applications thereof
AssigneeNational Central University
InventorsShaw-Jye WU, Lian-Chin WANG, Shin-Jye WU, Ching-Hui YEH, Chun-An LU
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Abstract
A method for providing thermotolerance of a plant and the genetic engineering applications thereof are disclosed. DNA fragment containing a gene encoded EXPORTIN1A is transferred into a plant cell to provide or enhance thermotolerance of the plant. The method can be applied in genetic engineering to select transgenic plant.
Claims20
A plant, plant cell, plant material or seed of a plant comprising:
- a recombinant vector construct introduced in the plant, the plant cell, the plant material or the seed of the plant, wherein the recombinant vector construct comprises:
- a promoter;
- and
- an isolated nucleic acid sequence operably linked to the promoter for expressing an exogenous XPO1A protein in the plant, the plant cell, the plant material or the seed of the plant, wherein the isolated nucleic acid sequence encodes an XPO1A protein of SEQ ID NO:
- 11,
- wherein the plant, the plant cell, the plant material or the seed of the plant that expresses the exogenous XPO1A protein has basal thermotolerance under long-term high temperature stress and sudden heat shock condition.
3 more elements
A method of producing a transformed plant cell, the method comprising:
- introducing into a plant cell a polynucleotide that encodes an exogenous XPO1A protein of SEQ ID NO:
- 11;
- and
- expressing the exogenous XPO1A protein in the plant cell,
- wherein the plant cell that expresses the exogenous XPO1A protein has basal thermotolerance..
2 more elements
A method of producing a transformed plant, the method comprising:
- introducing into a plant cell a polynucleotide that encodes an exogenous protein and an exogenous XPO1A protein of SEQ ID NO:
- 11;
- expressing the exogenous protein and the XPO1A protein in the plant cell;
- regenerating a whole plant from the plant cell at a survivable temperature;
- subjecting the transgenic plants to a sustained high temperature or a sudden heat shock treatment, wherein a sustained high temperature or a heat shock temperature is equal to or higher than a lethal temperature of a non-transgenic plant;
- and
- selecting the transgenic plant survived after sustained high temperature or sudden heat shock treatment,
- wherein the survived transgenic plant expresses the exogenous protein and the exogenous XPO1A protein under sustained high temperature or sudden heat shock condition.
5 more elements
Dependencies are inferred from the claim text.