Methods & Compositions for Selection of Loci for Trait Performance & Expression
OwnerWayne Kennard, Arnold Rosielle +4inventors
InventorsWayne Kennard, Arnold Rosielle, David Butruille, Sam Eathington, Kevin Cook, Trevor Hohls
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Abstract
The present invention provides novel methods and compositions for the identification and selection of loci modulating transgene performance and expression in plant breeding. In addition, methods are provided for screening germplasm entries for the performance and expression of at least one transgene.
Claims24
A method of identifying a plant germplasm entry with a genotype that modulates a performance of a transgenic trait, the method comprising:
- crossing at least two germplasm entries with a test germplasm entry comprising at least one transgenic trait;
- evaluating the performance of at least one transgenic trait in a progeny of each cross.
A plant, seed or part thereof containing at least one genomic region identified to modulate the performance of a transgenic event.
A plant, seed, or part thereof containing at least one genomic region for an enhanced performance of two or more transgenes.
A method of introgressing at least one transgene modulating locus into a plant comprising
- crossing at least one first plant with at least one second plant in order to form a population,
- genotyping at least one plant in the population with respect to a genomic nucleic acid marker selected from the group SEQ ID NOs:1-176, and selecting from the population at least one plant comprising at least one nucleic acid molecule selected from the group consisting of SEQ ID NOs:
- 1-176.
The method of claim 36, wherein the genotype is determined by an assay which is selected from the group consisting of single base extension (SBE), allele-specific primer extension sequencing (ASPE), DNA sequencing, RNA sequencing, microarray-based analyses, universal PCR, allele specific extension, hybridization, mass spectrometry, ligation, extension-ligation, and Flap Endonuclease-mediated assays.
The method of claim 36, further comprising the step of crossing the plant selected in step (c) to another plant.
The method of claim 36, further comprising the step of obtaining seed from the plant selected in step (c).
An elite plant produced by:
- a) crossing at least one first plant comprising a nucleic acid molecule selected from the group consisting of SEQ ID NOs:1-176 with at least one second plant in order to form a population,
- b) genotyping at least one plant in the population with respect to a genomic nucleic acid marker selected from the group SEQ ID NOs:
- 1-176, and
- c) selecting from the population at least one plant comprising at least one nucleic acid molecule selected from the group consisting of SEQ ID NOs:
- 1-176.
A substantially purified nucleic acid molecule for the detection of transgene modulating loci comprising a nucleic acid molecule selected from the group consisting of SEQ ID NOs:1-176 and complements thereof.
Dependencies are inferred from the claim text.