Nucleic acid purification with a binding matrix
AssigneePromega Corporation
InventorsRex M. Bitner, Michelle M. Mandreker, Paula R. Brisco
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Abstract
The present invention relates to methods, kits, and compositions for generating purified RNA samples and purified DNA samples. In particular, the present invention provides methods for generating a purified RNA or DNA sample from a sample containing both DNA and RNA molecules using a binding matrix that preferentially binds DNA or RNA in the presence of an acidic dilution buffer, or using a binding matrix that comprises acid zeolites, as well as compositions and kits for practicing such methods.
Claims36
A method of generating a purified DNA sample from an initial sample that comprises DNA and RNA molecules, said method comprising;
- a) contacting said initial sample with;
- i) a dilution buffer with an acidic pH, and ii) a nucleic acid binding matrix that preferentially binds RNA molecules in the presence of said dilution buffer;
- and
- b) separating said RNA-bound binding matrix from said initial sample the remaining initial sample thereby generating a purified DNA sample comprising a plurality of DNA molecules.
A method of generating a purified RNA and purified DNA sample from an initial sample that comprises DNA and RNA molecules, said method comprising;
- a) contacting said initial sample with;
- i) a dilution buffer with an acidic pH, and ii) a nucleic acid binding matrix, which comprises a zeolite, that preferentially binds both single and double stranded DNA molecules in the presence of said dilution buffer, wherein said contacting generates a DNA-bound binding matrix which is substantially RNA free;
- and
- b) physically separating said DNA-bound binding matrix from said initial sample the remaining initial sample now being a purified RNA sample comprising a plurality of RNA molecules;
- c) contacting the DNA-bound binding matrix with an elution buffer;
- and
- d) eluting DNA bound to the DNA-bound binding matrix thereby generating a purified DNA sample comprising a plurality of DNA molecules.
Dependencies are inferred from the claim text.