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US 2012/0059156 A1Published application
Method of protein nanostructure fabrication
AssigneeImiplex LLC
InventorsF. Raymond Salemme, Patricia C. Weber
Life of this patent
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Abstract
A method of assembling a protein nanostructure on a surface including selectively patterning a surface with a fixation site, bringing a protein node into contact with the surface, and allowing the protein node to bond with the fixation site, so that the position and/or orientation of the protein node is constrained, and compositions.
Claims33
A method of assembling a protein nanostructure, comprising:
- providing at least one protein node;
- selectively patterning a surface with at least one fixation site, bringing the at least one protein node into contact with the surface, and allowing the at least one protein node to bond with the fixation site, so that the position and/or orientation of the at least one protein node is constrained, or
- wherein the at least one protein node has a binding domain and a bound Ig (immunoglobulin), reacting a streptavidin or streptavidin derivative with a thiol-reactive azido-ATP reagent to form an azido-ATP modified streptavidin, allowing an Ig (immunoglobulin) and a dimeric ATP-binding protein incorporating a binding domain to interact to form a first complex, photocrosslinking the first complex with the azido-ATP modified streptavidin to form a second complex, and allowing the second complex to bind with the at least one protein node.
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A protein nanostructure, comprising:
- a protein node;
- and
- a selectively patterned surface having at least one fixation site, the protein node bonded with the at least one fixation site or
- a binding domain comprising a sequence of amino acid residues, the protein node comprising a plurality of subunits, the subunits covalently linked through a sequence of amino acid residues, and the binding domain covalently linked to a subunit.
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A kit, comprising:
- a selectively patterned solid surface having at least one fixation site;
- and
- a protein node,
- wherein the protein node can bond with the at least one fixation site.
1 more element
Dependencies are inferred from the claim text.