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US 2012/0059155 A1Published application
Method of Controlling O-Linked Glycosylation of Antibodies
AssigneeNovozymes Biopharma DK A/S
InventorsLeslie Robert Evans, Miranda Hughes, Joanna Hay, Darrell Sleep, David John Tooth, Neil Dodsworth, Malcolm John Saxton, Joanne Patricia Waters, Steven Athwal
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Abstract
A method for producing antibodies in fungal host cells is provided where the produced antibodies has a low degree of glycosylation.
Claims11
A method for preparing a polypeptide comprising an antibody sequence, said polypeptide having a low degree of O-linked glycosylation, comprising the step of:
- a. providing a nucleic acid sequence encoding a polypeptide comprising an antibody sequence;
- b. modifying the nucleic acid sequence so that at least one amino acid residue selected among S, T and Y and subjected to O-linked glycosylation is substituted or deleted;
- c. introducing the modified nucleic acid sequence in a suitable host cell so that the modified nucleic acid sequence is capable of being expressed in the host cell;
- d. growing the host cell under conditions leading to expression of the polypeptide encoded by the modified nucleic acid sequence, and
- e. recovering the polypeptide.
2 more elements
Dependencies are inferred from the claim text.